LN Ultra: Cryo Attachment for PowerTome
Cryo ultramicrotomy sectioning of both biological and industrial materials can be achieved with the LN Ultra, a liquid nitrogen-cooled cryo-chamber attachment for the PowerTome series of ultramicrotomes.
Manufacturer
RMC Boeckeler
Boeckeler Instruments engineers sample preparation equipment for nanoscale research, under the RMC Boeckeler product line. Their solutions are employed in both life sciences and materials research, with special emphasis in sample preparation for 3D electron microscopy.
Key Features
- Newly designed specimen arm with zero coefficient of thermal expansion using Invar alloy and high-efficiency copper heat sink
- Solid aluminium chamber provides greater thermal stability while providing a large opening for easy access to the specimen
- Multiple chamber heaters eliminate exterior condensation
- New pump with updated valves delivers liquid nitrogen in less time
- Brighter LED lights increases visibility in the chamber
- Re-designed cryo workstation provides comfort and stability when handling sections inside the chamber
- Produces ultra-thin cryosections for biological & materials applications
- Highest thermal stability for demanding Tokuyasu immunolabelling & CEMOVIS techniques
- Ideal for sectioning & cryo-planning co-polymer structures and nano-bio materials
- Excellent for SEM, AFM, and other scanning probe microscopies
- Quick install & easy removal for switching efficiently from cryo to room temperature sectioning and back
- Quickly switch between pre-cooled knives in holders designed to accommodate multiple diamond or glass knives, or a trim tool
- Knife clearance angle easily adjusted inside cooled chamber, with external knife rotation & lateral movement control
- Hybrid control system including touch screen computer and digital tactile controller when mounted on a PowerTome PC
- Rapid trimming with computer control
- Compact digital tactile controller displays knife and specimen temperatures, conveniently stacks on top of PowerTome controller
Overview
Rapid freezing and Freeze Substitution are one method of making cellular structure and organisation appear closer to the natural state but this process can limit other avenues of investigation, such as immunohistochemical applications.
Dehydration of a sample, necessary for routine electron microscopy of biological tissue, can be a major source of artefact and in many cases inhibits antibody-antigen reactions essential for immunolabelling/gold labelling studies. If a method could be found to preserve the water content in the cell during the process, this would greatly increase the scope of investigations, and the work Professor K.T. Tokuyasu provided for this breakthrough.
Today, cryosectioning and labelling of tissues and cell cultures by the ‘Tokuyasu Technique’ is a routine application in many laboratories.
Cryo ultramicrotomy sectioning of both biological and industrial materials can be achieved with the LN Ultra, a liquid nitrogen-cooled cryo-chamber attachment for the PowerTome series of ultramicrotomes. Mounted onto the PowerTome, the LN Ultra provides optimal thermal stability necessary for producing cryosections.
Capable of holding temperatures from +40°C to -185°C, the stable environment of the LN Ultra is perfect for sectioning and planning a variety of samples. These may include rubber and soft polymers, or biological cells and tissues; used for example, for immunolabelling using the Tokuyasu technique or CEMOVIS (Cryo-Electron Microscopy of Vitreous Ice Sections).
Liquid Nitrogen consumption is very low, allowing for a size efficient 15.5-litre dewar, capable of providing a stable sectioning environment for up to nine hours, depending on your technique and application, thus saving your laboratory LN2 costs over less efficient systems.
Specifications
| Temperature Range | +40°C to -180°C |
| Temperature Stability | +/- 0.1°C |
| LN2 Dewar Volume | 15.5L |
| Operating Time | Up to or over 9 hours |
Downloads
LN Ultra Datasheet
Service Support
Comprehensive repairs and servicing
Annual Support Programs
Your metrology instrumentation is a major investment that is critical to your business operation and success. In today’s competitive climate, it is more important than ever to extend the functionality and peak performance of your metrology equipment years beyond the expiration of your factory warranty. CN Tech’s Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.
With over 20 years’ experience servicing and repairing you can be assured that your system is in safe hands. The independent services we offer include system relocation, maintenance visits, parts and consumables, and break down interventions.
CN Tech’s Support Programs are an economical way to guarantee optimal working condition:
- Annual Preventive Maintenance
- Priority Technical Assistance
- Preferred Parts Availability
- On-Site User Training
- Remote Diagnostics
- No surprise repair expense and much more!
Support Contact Example
An example of our service and support contracts are shown below:
Metrology & Instrumentation Annual Support Programs 2022/23
CN Tech's Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.