BI-4500 Series
The 5-channel Surface Plasmon Resonance (SPR) instrument, combined with the BI Autosampler, is the ideal solution for high throughput assay development. With the BI-DirectFlow™ technology, it provides precise sampler delivery and high quality binding kinetic measurements.
Manufacturer
Biosensing Instrument
Biosensing Instrument (BI) provides biophysical tools to accelerate drug discovery research. They offer the traditional Surface Plasmon Resonance (SPR) tools which provides easily interchangeable application modules that enables liquid-phase SPR, gas-phase SPR, and electrochemical-enhanced SPR studies all on one instrument. The award winning SPR microscopy (SPRm) instrument integrates optical microscopy with SPR technology allowing the simultaneous study of label-free binding kinetics of membrane proteins with whole cells.
Surface Plasmon Resonance Explained
Key Features
- High throughput with 5 channels and fully automated autosampler - Provides 5 channel flow modes and high sample capacity with up to two 384 well plates. Temperature controlled sample storage from 4°C up to 20°C
- High sensitivity - Delivers high quality binding response for low immobilisation and small molecule (< 100 Daltons) detection
- Precise sample delivery with BI-DirectFlow™ Technology - Delivers sample with near-zero dispersion for fast kinetics and effective removal of various secondary effects
- Temperature controlled SPR detection module - Covers wide SPR angle range for measuring SPR shift in liquid phase. Precision background and reference subtraction. Temperature control for thermodynamic studies, 50 °C to 10 °C below room temperature (minimum 6°C)
- Innovative multi-module design - Provides users with maximum flexibility to choose amongst various analysis modules for electrochemistry and liquid applications
Base Station
| Light Source | Two low coherent light sources |
| Detection Speed | 4 ms |
| Incident Angles | 40-47 Deg (gas) 67-81 Deg (liquid) |
| Refractive Index Ranges | 1.00 - 1.40 |
| Baseline Noise | <0.06 RU RMS (0.01 mDeg RMS) |
| Baseline Drift | 0.30 RU/hr (0.05 mDeg/hr) (when ambient drifts <1°C/hr) |
| Measurement Ranges | Association rate constant ka: up to 108 M-1 s-1 Dissociation rate constant kd: 10-6 to 1 s-1 KD: 1pM to 1mM Active Concentration: 1pM to 1mM |
| Molecular Weight Cut-off | 100 Da |
| Temperature Control | Control accuracy at 0.01°C Range: 50°C to 10°C below ambient temperature max down to 6°C |
| PC Interface | USB 3.0 |
| Data IO Port | IO ports for sending and receiving raw signals with other equipment |
| Outer Dimension and Weight | 355 (w) x 250 (h) x 515 (d) mm and 11.5kg |
| Power Supply | 110-230 V 50/60 Hz |
Fluid Handling
| Sample Channels | 5 channels |
| Flow Cell Material | PEEK (biologically compatible) |
| Flow Rate | 1.0 to 150μL/min (application dependent) |
| Buffer Handling | Dual syringes driven by programmable infusion pump Providing >8 hours of continuing operation |
| Sample Injection Volume | 10 to 500 μL (application dependent) |
| Sample Injection Methods | Semi automated (BI-4500) Fully automated (BI-4500A) |
| Channel Volume | <32 nL |
| Injection Rise Time | <0.2 s |
| Analysis Module (standard) | 5-channel BI-DirectFlowTM Module |
| Optional Modules | EC-SPR module for SPR measurement with electrochemistry EC-DualFlowTM for two channel flow through EC-SPR measurement Gas SPR module for chemical vapour SPR measurement |
Control System
| Computer Software | Windows OS; SPR control software for real-time instrument control with programmable interaction assays for various application protocols; data analysis software for affinity, kinetics analysis, and concentration determination (calibrated or calibration-free) |
| Compliance | CE compliance; 21 CFR part 11 compliance; IQOQ certification |
Optional Accessories
| BI-Autosampler PP100 (standard with BI-4500A) | Automatic sample loading up to 768 compounds with programmable sample mixing and dilution. Sample capacity: two SBS microtiter plates or two 48 1.5ml Vials trays or two 12 10ml Vials trays |
| BI-auto-buffer exchange pump (standard with BI-4500AP) | Six ports auto buffer exchange pump with inline degasser extending continuing unattended operation >120 hours |
Applications
Life Science
Our Flow injection technique and analysis modules can help you with drug discovery and development, protein-protein/ protein-DNA/ protein-drug interaction studies, gene assays, immunosensing, and more.
Electrochemistry
Our Electrochemical SPR technique and analysis modules can help you with a variety of electro-analytical applications such as anodic stripping analysis, electropolymerization, electrodeposition, redox-related conformational change, and more.
Chemical Vapour Sensing
Our Gas Phase SPR technique and analysis modules can help you study fundamental solid-gas interface interactions, detect toxic molecules in the environment, perform various chemical analysis for medical research, and more.
Environment, Food & Beyond
Our BI SPR techniques can help you with broader applications such as food safety, quality control, environmental monitoring, or the development of new high-performance and coupled SPR techniques.
Downloads
BI-4500 Brochure
Environment, Food & Beyond
Chemical Vapour Sensing
Electrochemistry
Life Science
Service Support
Comprehensive repairs and servicing
Annual Support Programs
Your metrology instrumentation is a major investment that is critical to your business operation and success. In today’s competitive climate, it is more important than ever to extend the functionality and peak performance of your metrology equipment years beyond the expiration of your factory warranty. CN Tech’s Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.
With over 20 years’ experience servicing and repairing you can be assured that your system is in safe hands. The independent services we offer include system relocation, maintenance visits, parts and consumables, and break down interventions.
CN Tech’s Support Programs are an economical way to guarantee optimal working condition:
- Annual Preventive Maintenance
- Priority Technical Assistance
- Preferred Parts Availability
- On-Site User Training
- Remote Diagnostics
- No surprise repair expense and much more!
Support Contact Example
An example of our service and support contracts are shown below:
Metrology & Instrumentation Annual Support Programs 2024/25
CN Tech's Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.