SPRm 200 series
The cell-based Surface Plasmon Resonance Microscopy (SPRM) system, integrates optical microscopy and SPR, and it is a powerful technique for measuring binding activities of membrane proteins in vitro. It allows the simultaneous measurement of phenotypical changes of the sample via bright field and binding strength and kinetics via SPR.
Manufacturer
Biosensing Instrument
Biosensing Instrument (BI) provides biophysical tools to accelerate drug discovery research. They offer the traditional Surface Plasmon Resonance (SPR) tools which provides easily interchangeable application modules that enables liquid-phase SPR, gas-phase SPR, and electrochemical-enhanced SPR studies all on one instrument. The award winning SPR microscopy (SPRm) instrument integrates optical microscopy with SPR technology allowing the simultaneous study of label-free binding kinetics of membrane proteins with whole cells.
Surface Plasmon Resonance Microscopy
Key Features
- Integrated optical microscopy with SPR - Bright-field and SPR microscopies in one instrument. Large field of view with high resolution optics to view single or multiple cells
- In vitro and label-free binding activities mapping - Provides SPR sensor grams and binding activity map of individual cells as well as the binding kinetic constants (ka, kd, KD)
- Nanometer scaled binding response of virus, bacteria and nanoparticles - Nanomotion detection study of bacteria or virus metabolic activity or binding activities of nanoparticles for drug delivery designs
Base Station
| Light Source | 690 nm |
| Incident Angles | 40-76 Deg (continuous) |
| Baseline Noise | < 0.6 RU RMS (0.1 mDeg RMS) |
| Baseline Drift | 3 RU/hr (0.5 mDeg/hr) (when ambient drifts <1°C/hr) |
| Temperature Control Range | 15°C to 40°C (10°C below ambient temperature max) |
| Field of View | Bright Field: 1200 x 900 um SPR: 600 x 450 um |
| Magnification | Bright Field: x10 SPR: x20 |
| Resolution | Bright Field & SPR: 1 um |
| Stage Translation / Rotation | 3mm x 3mm / 360 deg |
| Outer Dimension | 690 (w) x 330 (h) x 340 (d) mm |
| Weight | 23kg |
| Power Supply | 110-230 V 50/60 Hz |
Fluid Handling
| Sample Volume | 1 to 1500 μL (application dependent) |
| Kinetic Constant | ka <1 X 107 M-1s-1 kd >1 X 10-5 s-1 |
| Dissociation Constant | KD = 10-3 M (1 mM) to 10-12 M (1 pM) |
| Molecular Weight Cutoff | 200 Da |
Control System
| Computer | Windows Operating System |
| Software | ImageSPRTM software including data analysis and kinetics analysis |
Autosampler (option)
| Sample Capacity | 2 x SBS Standards (384 / 96), 2 x 48 Vials (1.5ml), 2 x 12 Vials (10ml) |
| Sample Cooling | Minimum: 4°C +/- -2°C |
| Outer Dimension | 300 (w) x 575 (h) x 360 (d) mm |
| Weight | 21kg |
Automatic Buffer Exchange Pump and Degasser (option)
| Buffer Exchange | Automatic buffer exchange up to six sources |
| Buffer Degasser | In-line |
| Buffer Delivery | Continuous |
| Outer Dimension | 305 (w) x 191 (h) x 330 (d) mm |
| Weight | 6.8kg |
Downloads
SPRm 200 Series Brochure
Introduction of SPR Microscopy - Technical Note
Service Support
Comprehensive repairs and servicing
Annual Support Programs
Your metrology instrumentation is a major investment that is critical to your business operation and success. In today’s competitive climate, it is more important than ever to extend the functionality and peak performance of your metrology equipment years beyond the expiration of your factory warranty. CN Tech’s Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.
With over 20 years’ experience servicing and repairing you can be assured that your system is in safe hands. The independent services we offer include system relocation, maintenance visits, parts and consumables, and break down interventions.
CN Tech’s Support Programs are an economical way to guarantee optimal working condition:
- Annual Preventive Maintenance
- Priority Technical Assistance
- Preferred Parts Availability
- On-Site User Training
- Remote Diagnostics
- No surprise repair expense and much more!
Support Contact Example
An example of our service and support contracts are shown below:
Metrology & Instrumentation Annual Support Programs 2024/25
CN Tech's Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.