The interchangeable NIGHTSEA SFA Add-On Light and Filter Sets provide a Light Head, Barrier Filter and Filter Shield for viewing fluorescence of certain excitation and emission wavelengths. Each part is colour coded to easily match the correct excitation lights and emission filters. Each of the lights and filters are quick and easy to change.
Each NIGHTSEA SFA Add-On Light & Filter Set includes the following:
- A NIGHTSEA Light Head, which provides excitation light
- A NIGHTSEA Barrier Filter, to view fluorescence through the microscope
- A NIGHTSEA Filter Shield, for eye protection and enhanced viewing
- A protective case with foam insert to safely store the light head and filters
NIGHTSEA offer 6 different excitation and emission combinations to enable the NIGHTSEA fluorescence system to be used to view a comprehensive range of fluorophores. The available fluorescence combinations are:
- Royal Blue (RB)
- Excitation 440-460nm
- Emission 500nm (longpass filter)
- Green Only (RB-GO)
- Excitation 440-460nm
- Emission 500-560nm (bandpass filter)
- Cyan (CY)
- Excitation 490-515nm
- Emission 550nm (longpass filter)
- Green (GR)
- Excitation 510-540nm
- Emission 600nm (longpass filter)
- Violet (VI)
- Excitation 400-415nm
- Emission 460nm (longpass filter)
- Ultraviolet (UV)
- Excitation 360-380nm
- Emission 415nm (longpass filter)
The Green-Only Light & Filter Set provides a special barrier filter which isolates the green part of the spectrum and is used with the Royal Blue excitation source. The other NIGHTSEA filters are long-pass filters, while the Green-Only bandpass filter transmits from approximately 500 to 560 nm. The primary application of the Green-Only set is for viewing green-fluorescent protein (GFP) in plant specimens. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and image.
All current NIGHTSEA systems using NIGHTSEA Light Heads use a BNC connector to connect the Light Head. Some older NIGHTSEA systems use an alternative "V1" connector. The two different connector types are not interchangeable.
NIGHTSEA Excitation Lights and Emission Filters Specifications
Colour Code Filter Set Excitation Emission Fluorophores Dark Blue Royal Blue (RB) 440 - 460 nm 500nm LP GFP, eGFP, fluorescein,... Purple with "GO" Green Only (GO) 440 - 460 nm 500 - 560 nm BP GFP, eGFP, fluorescein,... Light Blue Cyan (CY) 490 - 515 nm 550 nm LP YFP, Venus,... Green Green (GR) 510 - 540 nm 600 nm LP DsRed, dTomato,... Purple Violet (VI) 400 - 415 nm 460 nm LP CFP,... Purple/Black Ultraviolet (UV) 360 - 380 nm 415 nm LP CFP,...
Each NIGHTSEA filter set is available as a complete set, including excitation light head, barrier filter and filter shield in a protective case. Excitation light heads, barrier filters and filter shields are also all available individually.
What customers think
I am very happy with the Nightsea, it serves its purpose for us well in genotyping/phenotyping Drosophila larvae expressing fluorescent proteins. A fraction of the cost of a conventional fluorescence dissecting microscope