ASH2: Advanced Substrate Holder Attachment for Serial Sectioning
For fast, reliable serial section collection for 3D tomography.
Manufacturer
RMC Boeckeler
Boeckeler Instruments engineers sample preparation equipment for nanoscale research, under the RMC Boeckeler product line. Their solutions are employed in both life sciences and materials research, with special emphasis in sample preparation for 3D electron microscopy.
ASH2 Installation
ASH2 Standard Knife Operation
ASH2 Jumbo Knife Operation
Key Features
- Holds substrates of various materials and sizes for ultrathin section collection
- 3-axes of movement allows for precise alignment of the substrate
- Ideal for the collection of hundreds of sections per substrate
- Can be used with standard room temperature diamond knives or jumbo knives
- Compatible with all current RMC and Leica ultramicrotomes
- Used to efficiently collect and align ribbons of serial sections
Overview
Advanced 3D reconstruction and tomography are growing in use in today’s EM world. For small volumes, where tens to hundreds of sections are required, the Advanced Substrate Holder (ASH2) is favoured for ease of use and speed.
The ASH2 is a multi-axis manipulation tool designed to precisely position substrates for the collection of ribbons of serial sections for array tomography. Standard room temperature knives and jumbo diamond knives for larger substrates can be used. Collected sections are subsequently imaged in the SEM or LM.
The ASH2 quickly mounts onto any current ultramicrotome, instantly converting it into an instrument capable of sectioning and collecting hundreds of sections.
This newly designed Advanced Substrate Holder (ASH2) weighs less than 0.5kg and can be mounted directly onto modern ultramicrotomes without modification or the use of additional fixtures. For larger volume work, please see our ATUMtome system, which is capable of effortlessly collecting thousands of sections.
3D Tomograms
Tomograms are produced by array tomography using serial sections from a sample of mouse renal cortex. Bowman’s capsule outlining a glomerulus (green), adjacent tubulus (yellow), the glomerular basement membrane (magenta, blue), and glomerular cells (blue) with a background BSD “TEM-like” image from the final layer. *
*3D array prepared by: Murray C. Killingsworth PhD (1 – 4) and Tzipi Cohen Hyams PhD (1-3) as below:
- Ingham Institute for Applied Medical Research
- University of New South Wales, Sydney
- Western Sydney University
- New South Wales Health Pathology
Size
| Dimensions | 66mm x 74mm x 53mm |
| Net Weight | 0.5kg / 1 Ib |
Downloads
ASH2 Installation
ASH2 Standard Knife Operation
ASH2 Jumbo Knife Operation
ASH2 Glue Recommended Application
ASH2 Datasheet
Service Support
Comprehensive repairs and servicing
Annual Support Programs
Your metrology instrumentation is a major investment that is critical to your business operation and success. In today’s competitive climate, it is more important than ever to extend the functionality and peak performance of your metrology equipment years beyond the expiration of your factory warranty. CN Tech’s Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.
With over 20 years’ experience servicing and repairing you can be assured that your system is in safe hands. The independent services we offer include system relocation, maintenance visits, parts and consumables, and break down interventions.
CN Tech’s Support Programs are an economical way to guarantee optimal working condition:
- Annual Preventive Maintenance
- Priority Technical Assistance
- Preferred Parts Availability
- On-Site User Training
- Remote Diagnostics
- No surprise repair expense and much more!
Support Contact Example
An example of our service and support contracts are shown below:
Metrology & Instrumentation Annual Support Programs 2022/23
CN Tech's Support Programs will help ensure that your investment is protected, and that you and your instrument’s are always operating at peak performance.